{"id":843,"date":"2024-02-12T15:21:21","date_gmt":"2024-02-12T22:21:21","guid":{"rendered":"https:\/\/ackerson.colostate.edu\/?p=843"},"modified":"2024-02-12T15:21:21","modified_gmt":"2024-02-12T22:21:21","slug":"bradley-makayla-visit-the-new-york-structural-biology-center-to-do-single-particle-cryoem","status":"publish","type":"post","link":"https:\/\/ackerson.colostate.edu\/index.php\/2024\/02\/12\/bradley-makayla-visit-the-new-york-structural-biology-center-to-do-single-particle-cryoem\/","title":{"rendered":"Bradley &#038; MaKayla visit the New York Structural Biology Center to do Single Particle CryoEM!"},"content":{"rendered":"\n<p>Bradley and MaKayla visited the National Center for CryoEM Access and Training (NCCAT) within the Simons Electron Microscopy Center at the New York Structural Biology Center in New York, New York at the beginning of February. During their visit, they were screening samples of GRLMR with different small molecules\/substrates for single particle cryo-electron microscopy. Here, the goal was to screen samples to find grids suitable for long, automated data collection to obtain atomic or near-atomic 3-dimensional structures of GRLMR. Within these structures, they hope to see how these small molecules\/substrates are binding within the enzyme.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_5668-1-1024x768.jpg\" alt=\"\" class=\"wp-image-854\" srcset=\"https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_5668-1-1024x768.jpg 1024w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_5668-1-300x225.jpg 300w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_5668-1-768x576.jpg 768w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_5668-1-1536x1152.jpg 1536w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_5668-1-2048x1536.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>Here, MaKayla and Bradley stand near one of the Glacios Cryo-Electron microscopes after loading samples for overnight screening<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" src=\"https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_1268-1-768x1024.jpg\" alt=\"\" class=\"wp-image-856\" srcset=\"https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_1268-1-768x1024.jpg 768w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_1268-1-225x300.jpg 225w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_1268-1-1152x1536.jpg 1152w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_1268-1-1536x2048.jpg 1536w, https:\/\/ackerson.colostate.edu\/wp-content\/uploads\/2024\/02\/IMG_1268-1-scaled.jpg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure>\n\n\n\n<p>Here MaKayla manipulates a box of samples under liquid nitrogen towards the transfer station (black and white box) which will load up to 12 samples at a time, all constantly under liquid nitrogen.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bradley and MaKayla visited the National Center for CryoEM Access and Training (NCCAT) within the Simons Electron Microscopy Center at the New York Structural Biology Center in New York, New York at the beginning of February. During their visit, they were screening samples of GRLMR with different small molecules\/substrates for single particle cryo-electron microscopy. Here, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":851,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-843","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/posts\/843","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/comments?post=843"}],"version-history":[{"count":1,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/posts\/843\/revisions"}],"predecessor-version":[{"id":857,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/posts\/843\/revisions\/857"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/media\/851"}],"wp:attachment":[{"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/media?parent=843"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/categories?post=843"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ackerson.colostate.edu\/index.php\/wp-json\/wp\/v2\/tags?post=843"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}